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2 edition of Biochemical and serological characterisation of a calf rotavirus found in the catalog.

Biochemical and serological characterisation of a calf rotavirus

Helen Killen

Biochemical and serological characterisation of a calf rotavirus

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Published by [typescript] in [s.l.] .
Written in English


Edition Notes

Thesis (Ph.D.) - University of Warwick, 1983.

Statementby Helen Killen.
ID Numbers
Open LibraryOL14872843M

Rotavirus vaccines are very effective at preventing rotavirus disease. Infants should get either of the two available rotavirus vaccines: RotaTeq® is given in 3 doses at ages 2 months, 4 months, and 6 months; Rotarix® is given in 2 doses at ages 2 months and 4 months.


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Biochemical and serological characterisation of a calf rotavirus by Helen Killen Download PDF EPUB FB2

Biochemical and serological characterisation of a calf rotavirus. Author: Killen, H. ISNI: Awarding Body: University of Warwick Current Institution: University of Warwick Date of Award: Availability of Full Text: Access from EThOS.

Antigenic and biochemical characterization of bovine rotavirus V, a new member of rotavirus serotype 10 Harald Briissow, 1. a serological and biochemical description of BRV V (Bachmann & Hess, ), which has been characterized calf rotavirus.

CLIN. MICROBIOL. TABLE 1. Serologic characterization ofbovine rotaviruses from dairy andbeefherds in Argentina byusing IPFRA Rotavirus Titerb ofhyperimmune antiserum to rotavirus: strain Orlgln UK NCDV B B T21 T26 T82 UK United Cited by: Further biochemical characterization, including the detection of surface glycoproteins, of human, calf, and simian rotaviruses November Journal of Virology 24(1)   Bovine rotavirus T was isolated from feces of a calf with diarrhea.

Serological characterization by serotype-specific monoclonal antibodies showed that the T virus belonged to serotype 1. This is the first report of a bovine rotavirus that does not belong to serotype 6, 8, or Cited by: Polypeptides from purified virions of the calf rotavirus (RV) isolate /83 and those from the pigeon RV present report we extend the biochemical and serological Characterization of basic growth parameters and pattern of macromolecular synthesis.

Journal of Virol laboratory workers trained in methods for rotavirus detection and characterization. Although rotavirus diagnostic procedures are routine, characterization methods include a variety of specialized techniques for the antigenic and molecular identification of rotavirus strains.

A compendium of current methods and protocols and a general strategy for. If the calf is not drinking, stomach tubing is indicated.

If the calf collapses then intravenous fluids should be given. Antibiotics: The use of antibiotics to treat calf diarrhoea is controversial, particularly if rotavirus is the main cause.

Antibiotics will not kill the virus, but they can reduce secondary bacterial infection. Fourteen strains of human rotaviruses were propagated in MA cell culture using serum-free medium containing 10 µg/ml trypsin.

Eleven of the strains were provided by laboratories in Japan and the U.S.A., and the remaining three from our own laboratory. The serological relationships between these strains were determined by rotavirus subgroup-specific enzyme-immunoassays (ELISA) and.

Rotavirus is the biggest animal health issue facing calf rearers with some experiencing death rates as high as 30%. It can be devastating and demoralising. Rotavirus is persistent in the environment, and can remain infectious for many months at room temperature. It can withstand low temperatures and.

In this study, a novel bovine group A rotavirus (BoRV-A), Sun9, isolated from calf diarrhea in Tochigi Prefecture, Japan, was serologically characterized by a cross-neutralization assay, and serological surveillance by using its reassortant was performed on cattle bred in Japan.

Identification of four VP4 serological types (P serotypes) of bovine rotavirus using viral reassortants October Journal of General Virology 73 (Pt 9)(9) Summary. Human, piglet, mouse, foal, lamb, calf and rabbit rotaviruses all infected, but could not readily be subcultured in LLC MK 2 cells.

Cells infected with mouse and calf rotaviruses reacted by indirect immunofluorescence (FA) with convalescent serum from children, piglets, mice, foals, lambs, calves or rabbits, taken after rotavirus infection.

Serological characterization by serotype-specific monoclonal antibodies showed that the T virus belonged to serotype 1.

This is the first report of a bovine rotavirus that does not belong to. Pink Book’s Chapter on Rotavirus pdf icon [12 pages] Epidemiology & Prevention of Vaccine-Preventable Diseases textbook.

Publications MMWR. Hallowell BD, Parashar UD, Curns A, DeGroote NP, Tate JE. Trends in the Laboratory Detection of Rotavirus Before and After Implementation of Routine Rotavirus Vaccination — United States, – Serological profile revealed increase in chloride level (%; SD: ; 95% CI: ) in rotavirus positive diarrheic calves as compared to rotavirus negative cases and suggested the.

Rotavirus Infection: Few ,many out Young animals are disease sponges and disease amplifiers Unique Features of Rotavirus •Rotavirus is –Everywhere. –Resistant to most disinfectants • Things that will inactivate rotavirus –A torch –A pH below 2 (battery acid.

Introduction. Rotavirus (RV) is the principal etiological agent of severe gastroenteritis (GE) in young children worldwide (Parashar et al., ).Despite their preferential replication in the intestine, RV induces both an intestinal and systemic immune response, the latter most probably related to the antigenemia and viremia present in acutely infected children and animals (Blutt and Conner.

ELSEVIER Veterinary Microbiology 42 () veterinary microbiology Serological and genotypic characterization of group A rotavirus reassortants from diarrheic calves born to dams vaccinated against rotavirus 1 Wei Lu a, Gerald E. Duhamel a,*, David A. Benfield b, Dale M. Grotelueschen c a Department of Veterinary and Biomedical Sciences, University of Nebraska.

Bridger JC, Woode GN. Characterization of two particle types of calf rotavirus. J Gen Virol. May; 31 (2)– Cardiff RD, Puentes MJ, Teramoto YA, Lund JK. Structure of the mouse mammary tumor virus: characterization of bald particles.

J Virol. Nov; 14 (5)– [PMC free article]. A Longitudinal Cohort Study in Calves Evaluated for Rotavirus Infections from One to 12 Months of Age by Sequential Serological Assays May Archives of Virology (5) of rotavirus (G1–4, G9) accounted for 90% of isolates from children younger than 5 years in the United States.

Of these, the G1 strain accounted for more than 75% of isolates. Rotavirus is very stable and may remain viable in the environment for weeks or months if not disinfected. Rotaviruses cause infection in many species of mammals.

not cause diarrhea in experimental calf infection, but serological surveys and isolation studies showed that the virus was a common full biochemical characterization of the virus and its antigenic Viruses are found commonly in association with neonatal calf diarrhea: rotavirus and coronavirus are being associated with % of.

An atypical group A rotavirus (/83) was isolated from a 3-day-old German calf with diarrhea. It differed from 35 conventional German bovine rotavirus isolates analyzed previously with respect to subgroup (strain /83 was non-subgroup I and non-subgroup II), serotype (strain /83 showed a two-way cross-reaction with serotype 7 and a one-way cross-reaction with serotype 3), and.

differing by their antigenic, biochemical or biological properties (5, 10, 16, 32, 36, 56, 63) and sometimes by their pathogenicity (3, 4, 5).

The dose of virus is also an important factor: diarrhoea was observed in piglets only when the amount of rotavirus increased in the surroundings (22) and a dose effect was also suggested for calves (2, 61). Purified virions or radiolabelled lysates of infected MA cells were used to characterize the structural and nonstructural polypeptides of a porcine group C rotavirus.

At least six structural proteins were identified from purified group C rotavirus by sodium dodecyl sulfate- polyacrylamide gel electrophoresis. Of these, two (37 and 33 molecular-weight polypeptides) were associated.

Characterization of the bovine group A rotavirus strain neonatal calf diarrhea virus-Cody (NCDV-Cody). W Lu, G E Duhamel, Y Hoshino, D A Benfield, E A Nelson, and R A Hesse Department of Veterinary and Biomedical Sciences, University of Nebraska-LincolnUSA.

A study was conducted to determine the occurrence of rotavirus in faeces of calves from 6 counties of the northeast region in the State of São Paulo, Brazil.

faecal samples ( and from diarrhoeic and healthy animals, respectively) were collected from calves days old between June and March The techniques used for the detection of rotavirus were enzyme immunoassay (EIA).

Helen Killen has written: 'Biochemical and serological characterisation of a calf rotavirus' What three conditions of the serum can interfere with serological test. Icterus, Hemolysis, or Lipemia.

Abstract. The Rotavirus genus of the Reoviridae consists of a number of antigenically related and morphologically identical viruses that cause enteritis, primarily in young mammals and avians.

The genus was established by the International Committee on Taxonomy of Viruses at the Fourth International Congress for Virology in (Matthews ).Type species, such as human and bovine rotavirus. typing rotavirus have been described that should allow the specific characterization of human strains by biochemical or antigenic analyses [10, 11].

Third, attenuated rotaviruses have been dis-covered [6] or derived [12] and are being evaluated as vaccine candidates. In this issue of the Journal of Infectious Diseases, five studies are published.

Rotavirus was excreted in the faeces of the lambs for several days. One-day-old lambs were more susceptible than day-old lambs. A serological response to infection was detected in all lambs by complement fixation and immunofluorescence techniques, using antigens prepared from calf rotavirus.

Rodger SM, Schnagl RD, Holmes IH. Further biochemical characterization, including the detection of surface glycoproteins, of human, calf, and simian rotaviruses. J Virol. Oct; 24 (1)– [PMC free article] Rodger SM, Schnagl RD, Holmes IH.

Biochemical and biophysical characteristics of diarrhea viruses of human and calf origin. J Virol. pdfs/ Serological relation between calf diarrhea coronavirus and hemagglutinating encephalomyelitis pdfs/ Rotavirus Shedding in Feces of Gnotobiotic Calves Orally Inoculated with a Commercial Rotavirus pdfs/ Biochemical Characterization of the Equine Arteritis Virus Helicase Suggests a Close Functional.

In this study, serological and molecular research was conducted on the Rotavirus infection in domestic breeds of sheep at 2–3 years of age. The sheep included in the study were raised on small scale family units of less than 20 sheep per unit, in central Kars province and its districts (Susuz, Arpaçay, Kagizman and Selim) in the Northeast.

Serological relationships between rotaviruses from different species as studied by complement fixation and neutralization.

Arch Virol. ; 53 (4)– Rodger SM, Schnagl RD, Holmes IH. Biochemical and biophysical characteristics of diarrhea viruses of human and calf origin.

J Virol. Nov; 16 (5)– [PMC free article]. Standard biochemical tests for detection of E. coli were performed on EMB positive isolates according to Kreig and Holt () including indole production test, methyl - red test, Voges- Proskauer test, Simmon’s citrate test, triple sugar iron (TSI) and urease test.

Serological characterization of E. coli isolates. Serological and genomic characterization of L, a novel equine group A rotavirus G serotype.

J Gen Virol. May; 72 (Pt 5)– Browning GF, Fitzgerald TA, Chalmers RM, Snodgrass DR. A novel group A rotavirus G serotype: serological and genomic characterization of equine isolate FI J Clin Microbiol. Sep; 29 (9)– Diarrhea is common in newborn calves, lambs, and kids.

The clinical presentation can range from mild diarrhea without systemic disease to profuse, acute diarrhea associated with rapid dehydration, severe disturbance of acid-base and electrolyte balance, and death, sometimes in as few as 12 hr.

Characterization of two particle types of calf rotavirus. J Gen Virol. May; 31 (2)– Clark SM, Barnett BB, Spendlove RS. Production of high-titer bovine rotavirus with trypsin. J Clin Microbiol. Mar; 9 (3)– [PMC free article] Cohen J. Ribonucleic acid polymerase activity associated with purified calf rotavirus.

Many immunoassays have been developed for serological characterization of N. meningitidis antigens. Immunological detection of specific epitopes of two major outer-membrane proteins, PorB and PorA, is the basis for the serological classification system defining, respectively, the serotypes and serosubtypes of the species 1, ).Rodger SM, Schnagl RD, Holmes IH.

Further biochemical characterization, including the detection of surface glycoproteins, of human, calf, and simian rotaviruses. J Virol. Oct; 24 (1)– [PMC free article] Thouless ME. Rotavirus polypeptides. J Gen Virol.

Jul; 44 (1)–Middleton PJ, Holdaway MD, Petric M, Szymanski MT, Tam JS. Solid-phase radioimmunoassay for the detection of rotavirus.

Infect Immun. May; 16 (2)– [PMC free article] Rodger SM, Schnagl RD, Holmes IH. Further biochemical characterization, including the detection of surface glycoproteins, of human, calf, and simian rotaviruses.